Definition of novel cell envelope associated proteins in Triton X-114 extracts of Mycobacterium tuberculosis H37Rv

Comprehensive analysis of exported proteins from Mycobacterium tuberculosis H37Rv.

Proteins secreted by Mycobacterium tuberculosis play an essential role in the pathogenesis of tuberculosis. The culture filtrates of M. tuberculosis H37Rv made by Sadamu Nagai (Japan), are considerably enriched for secreted proteins compared to other culture filtrates. Complementary approaches were used to identify the secreted proteins in these culture filtrates: (i) 2-DE combined with MALDI-T...

HYPOTHETICAL PROTEINS OF MYCOBACTERIUM TUBERCULOSIS H37Rv AS DRUG TARGETS

Hypothetical proteins often with unknown functions . Mycobacterium tuberculosis H37Rv has a very large number of such proteins , which can be exploited as drug target as the bacterium became more virulent with developing antibiotic resistance to first line and second line of drugs . Some hypothetical proteins (290 proteins) were studied from different aspects related to find the druggable prote...

Tricarboxylic acid cycle and related enzymes in cell-free extracts of Mycobacterium tuberculosis H37Rv.

Survival mechanisms of pathogenic Mycobacterium tuberculosis H37Rv.

Mycobacterium tuberculosis H(37)Rv is a highly successful pathogen and its success fully relies on its ability to utilize macrophages for its replication and, more importantly, the macrophage should remain viable to host the Mycobacterium. Despite the fact that these phagocytes are usually very effective in internalizing and clearing most of the bacteria, M. tuberculosis H(37)Rv has evolved a n...

Phase separation of integral membrane proteins in Triton X-114 solution.

A solution of the nonionic detergent Triton X-114 is homogeneous at 0 degrees C but separates in an aqueous phase and a detergent phase above 20 degrees C. The extent of this detergent phase separation increases with the temperature and is sensitive to the presence of other surfactants. The partition of proteins during phase separation in solutions of Triton X-114 is investigated. Hydrophilic p...